Fluorescence Microscopy Now In-House at APS

December 9, 2016

With our recent acquisition of fluorescence microscopy, APS has added another powerful in-house pathology tool for the evaluation of your preclinical study. Unlike normal bright field (transmitted light) microscopy where tissue slide sections are primarily evaluated for changes in cellular morphology and tissue organization, fluorescence microscopy utilizes light signals produced by the sample itself to produce highly sensitive and selective visualization of labeled structures, cells and subcellular components.

Fluorescence microscopy techniques are expanding rapidly in the biological sciences. Some applications of potential interest in preclinical studies include the following:

  • Immunofluorescence microscopy: Immunofluorescence utilizes strongly fluorescing molecules to label antibodies and results in highly sensitive and highly selective visualization of targeted antigens. Immunofluorescence can be used in determining cell viability and localizing cell structural components, for example.
  • Fluorescent microbeads: Fluorescent labeled microbeads/microspheres used in flow or occlusion studies can be readily detected in routinely processed tissue sections with fluorescence microscopy.
  • Fluorescent molecules: Naturally fluorescing compounds that integrate into tissue can be used to evaluate tissue growth and repair. This method is most commonly used in bone studies where calcium binding fluorescent molecules integrate into mineralized bone.

Contact us at APS if your preclinical study has a need that could be addressed by the application of fluorescence microscopy.